PRP presented at Elite Meetings

See BloodCure.com for the details of platelet rich plasma presentations at elite meetings.

AM
Total Tendon

Review of PRP for Muscle and Tendon

Below is the abstract from a soon to be published article on the use of PRP for tendon and muscle. It will be published in a volume of Clinics in Sports Medicine entitled: "Future Trends in Sports Medicine"

AM (Total Tendon, ApexPRP)

Treatment of Tendon and Muscle Using Platelet-Rich Plasma.
Mishra A, Woodall J Jr, Vieira A.
Clin Sports Med. 2009 Jan;28(1):113-125.

Department of Orthopedic Surgery, Menlo Medical Clinic, Stanford University Medical Center, 1300 Crane Street, Menlo Park, CA 94025, USA.
Tendon and muscle injuries are common in elite and weekend athletes. Treatment of these injuries in both groups is rapidly evolving. Sports medicine patients are demanding better and less invasive solutions for all types of musculoskeletal disorders. In this context, platelet-rich plasma (PRP) has emerged as a potential solution. PRP is a fraction of whole blood containing concentrated growth factors and proteins. These cytokines direct tissue healing through autocrine and paracrine effects. The number of basic science, animal, and human investigations of PRP for tendon and muscle injuries worldwide has risen sharply in recent years. These studies are helping clinicians better understand the mechanisms of PRP and are guiding novel treatment protocols. In this paper, the value of PRP as a treatment for acute or chronic tendon and muscle disorders is explored.

Platelet Rich Plasma and Anterior Cruciate Ligaments

In an animal model, PRP did not improve suture repair of Anterior Cruciate Ligaments. (See abstract below) A still unanswered question is, does PRP improve ACL reconstruction surgery healing times or can PRP help heal a partial ACL tear. These studies have yet to be done.

AM

Total Tendon
ApexPRP

J.Ortho Research 2008

Platelet-rich plasma alone is not sufficient to enhance Suture Repair of the ACL in skeletally immature animals: An in vivo study.

Murray MM, Palmer M, Abreu E, Spindler KP, Zurakowski D, Fleming BC.

Department of Orthopaedic Surgery, Children's Hospital Boston, 300 Longwood Avenue, Boston, Massachusetts 02115.

In this study, we hypothesize that supplementation of suture repair of the anterior cruciate ligament (ACL) with platelet-rich plasma (PRP) will improve the biomechanics of the repair. Six 30-kg pigs underwent bilateral suture repair of the ACL. One side was treated with suture repair alone, while the contralateral side was treated with suture repair augmented with PRP. After 14 weeks in vivo, anterior-posterior (AP) knee laxity and the tensile properties of the repaired ligament were measured. The addition of PRP to the suture repairs did not improve AP knee laxity at 30 degrees (p = 0.73) or 60 degrees (p = 0.65). It also did not improve the maximum tensile load (p = 0.64) or linear stiffness (p = 0.42) of the ACL repairs after 14 weeks in vivo. The model had 80% power to detect a 30% improvement of biomechanical properties with PRP; thus, we are confident that a clinically meaningful effect as a result of adding PRP is unlikely. Use of PRP alone to supplement suture repair of the ACL is ineffective in this animal model. Published by Wiley Periodicals, Inc. J Orthop Res.

Cartilage Formation better with Platelet Rich Plasma

Platelet lysate favours in vitro expansion of human bone marrow stromal cells for bone and cartilage engineering.

Zaky SH, Ottonello A, Strada P, Cancedda R, Mastrogiacomo M.

Istituto Nazionale per la Ricerca sul Cancro, and Dipartimento di Oncologia, Biologia e Genetica dell'Universita' di Genova, Genova, Italy.

The heterogeneous population of non-haematopoietic cells residing in the bone marrow (bone marrow stromal cells, BMSCs) and the different fractions and components obtained from platelet-rich plasma provide an invaluable source of autologous cells and growth factors for bone and other connective tissue reconstruction. In this study, we investigated the effect of an allogenic platelet lysate on human BMSCs proliferation and differentiation. Cell proliferation and number of performed cell doublings were enhanced in cultures supplemented with the platelet-derived growth factors (platelet lysate, PL), either with or without the concomitant addition of fetal bovine serum (FBS), compared to cultures performed in the presence of FBS and FGF2. Both in vitro and in vivo osteogenic differentiation were unaltered in cells maintained in medium supplemented with PL and not FBS (Only PL) and in cells maintained in medium containing FBS and FGF2. Interestingly, the in vitro cartilage formation was more effective in the pellet of BMSCs expanded in the Only PL medium. In particular, a chondrogenic differentiation was observed in pellets of some in vitro-expanded BMSCs in the Only PL medium, whereas pellets from parallel cell cultures in medium containing FBS did not respond to the chondrogenic induction. We conclude that the platelet lysate from human source is an effective and even more beneficial substitute for fetal bovine serum to support the in vitro expansion of human BMSCs for subsequent tissue-engineering applications.

Total Tendon

Los Angeles Dodger Closer Returns after PRP

Read the story of how PRP may have helped heal an elbow ligament injury in LA Dodger Takashi Saito.

Video of PRP Basic Science

This is a video of the basic science of platelet rich plasma. Dr. Allan Mishra produced the video.



Total Tendon

BloodCure.com

PRP Presented at Las Vegas Meeting

Dr. Mishra presented his PRP research at the Advanced Concept Sports Medicine Meeting in Las Vegas last weekend.